Determine® HIV-1/2 SOP

Summary of the manufacturer’s working protocol for Determine® Rapid Tests. 

Procedure

  1. Check the expiration date. Do not use expired kits.
  2. Label test device with the appropriate patient/client identification.
  3. Remove the protective kit cover from the test device.
  4. Applyonedropofwholeblood(50ul)tothesamplepadmarkedbyan arrow
  5. Wait one minute until blood is absorbed into the sample pad.
  6. Apply1dropofChaseBuffertothesamplepad.
  7. Read the result after15 minutes.
  8. Record results on the worksheet

Interpretation of Test Results (Only three results are possible with this test): Reactive (Two bands)

Red bands appear in both the control window (labeled ‘C’) and the patient window (labeled ‘P’) of the strip. Any visible red color in the patient window should be interpreted as positive.

Non-reactive (One Band)
One red band appears in the control window of the strip (labeled ‘C’) and no

red band appears in the patient window of the strip (labeled ‘P’).

Invalid (No Band)

If there is no band in the control window of the strip the test is invalid. Even if a red band is present in the patient window of the strip, the result is invalid and should be repeated.